normal lung epithelial cell line beas 2b  (ATCC)

Journal: Translational Oncology

Article Title: THOC3 interacts with epithelial-to-mesenchymal transition to promote non-small cell lung cancer carcinoma progression through STAT3 signaling pathway

doi: 10.1016/j.tranon.2026.102741

Figure Lengend Snippet: THOC3 expression is upregulated in LC and is associated with unfavorable prognoses. (A, B) The mRNA expression of THOC3 in LUAD and LUSC tissues, as well as normal lung tissues, was obtained from the GEPIA database. (C) Real-time PCR analysis of THOC3 mRNA expression in cancerous and adjacent non-cancerous tissues from LC patients. (D) Western blot analysis of THOC3 protein levels in cancerous and adjacent tissues from LC patients. (E) Real-time PCR analysis of THOC3 mRNA expression in LC cell lines (H1975, A549, H1299, and PC9) and the normal bronchial epithelial cell line BEAS-2B (F) Western blot analysis of THOC3 protein expression in LC cell lines (H1975, A549, H1299, and PC9) and BEAS-2B cells. (G) Real-time PCR analysis of THOC3 mRNA expression in normal lung tissues and LC samples from different clinical grades. (H) Western blot analysis of THOC3 protein levels in normal lung tissues and LC samples from various clinical stages. (I, J, K) Kaplan-Meier survival curves illustrating the overall survival (OS) of LC patients with high or low THOC3 expression from the TCGA database. (L) Kaplan-Meier survival curves depicting disease-free survival (DFS) in high vs. low THOC3-expressing LC patients from the TCGA database. Each experiment was performed in triplicate. Data are expressed as mean ± SEM; * P < 0.05.

Article Snippet: The human normal lung epithelial cell line BEAS-2B was obtained from the American Type Culture Collection.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Frontiers in Oncology

Article Title: Acidic microenvironment-induced LAMC2 expression promotes proliferation, migration, and pathway regulation in non-small cell lung cancer cells

doi: 10.3389/fonc.2026.1747868

Figure Lengend Snippet: Acidic microenvironment condition screening and LAMC2 expression validation. (A) Comparison of relative LAMC2 expression levels in different cell lines (BEAS-2B, HCC827, and A549), showing a significantly higher expression in HCC827 and A549 cells compared to BEAS-2B cells. (B) Changes in LAMC2 expression in HCC827 and A549 cells under different lactate (LA) concentrations (10, 20, and 30 mM), showing significant increases in LAMC2 expression at 20 and 30 mM concentrations. (C) Changes in LAMC2 expression over time (0, 6, 12, and 24 h) in HCC827 and A549 cells treated with 20 mM LA, showing a significant increase in expression with longer treatment times. (D) Changes in LAMC2 expression under different acidic environment conditions (high LA, hydrochloric-acid-induced acidosis, and control groups) in HCC827 and A549 cells, with a significantly higher expression in the high LA and hydrochloric-acid-induced acidosis groups. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Human normal lung epithelial BEAS-2B cells (ATCC ® CRL-9609TM) and lung cancer cell lines A549 (Procell, CL-0016, lung adenocarcinoma) and HCC827 (Procell, CL-0094, lung adenocarcinoma) were obtained from the indicated suppliers.

Techniques: Expressing, Biomarker Discovery, Comparison, Control

Journal: Clinical and Experimental Medicine

Article Title: PRKD1/EIF5A/β-catenin axis inhibits tumorigenesis and metastasis in non-small-cell lung cancer

doi: 10.1007/s10238-026-02068-9

Figure Lengend Snippet: Expression and prognosis analysis of PRKD1 in NSCLC. ( A ) The expression of PRKD1 in NSCLC tissues compared with normal lung tissues was analyzed by GSE10072 , GSE19804 and GSE32863 datasets. ( B ) The expression of PRKD1 in NSCLC tissues compared with normal lung tissues was analyzed by the GEPIA datasets. ( C ) Overall survival and first progression survival between low and high PRKD1 expression were analyzed by the Kaplan–Meier plotter database. ( D ) Expression of PRKD1 was compared between stage I-II and stage III-IV NSCLC patients from the TCGA database. ( E ) Gene expression of PRKD1 in normal lung epithelial cells BEAS-2B and four NSCLC cells was detected by qRT-PCR. All bar plot data are the means ± SD of three independent experiments

Article Snippet: NSCLC cell lines H1975, PC9 and normal lung epithelial cell line BEAS-2B were purchased from Procell Life Science & Technology Co., Ltd (Wuhan, China).

Techniques: Expressing, Gene Expression, Quantitative RT-PCR